[Abstract]Objective:To investigate the effect of low-dose dexmedetomidine on the stress response during tracheal extubation after general anesthesia and laparoscopic surgery, and investigating its safety and feasibility in anesthesia extubation. Method: Sixty patients with ASA grade I-II gynecological surgery requiring general anesthesia and tracheal intubation were selected for selection. According to the random number table method, the enrolled patients were divided into two groups: the control group (group C) and the dexmedetomidine group (group D), with 30 cases in each group. The anesthesia induction and maintenance medication regimen for the two groups of patients is the same. Anesthesia induction was performed by intravenous injection of sufentanil, propofol, and rocuronium bromide. Anesthesia was maintained by target control with propofol and remifentanil, inhalation of sevoflurane, and intermittent administration of rocuronium bromide as needed. Before the surgery is completed, a single dose of 0.05mg/kg or an equal volume of physiological saline should be administered intravenously. When the indication for extubation is reached after the surgery, remove the tracheal tube. Record the incidence and severity of coughing during tracheal extubation in two groups of patients, changes in mean arterial pressure (MAP), heart rate (HR), and pulse oxygen saturation (SpO2) before and after extubation, anesthesia recovery time, extubation time, post extubation sedation score, and complications.Results: The severity and incidence of coughing in group D were significantly reduced (23% vs 77%), and the difference between the two groups was statistically significant (p0 05). There was no significant difference inMAP values between groups during extubation and within groups (P0.05), while HR values increased significantly in group C compared to group D during extubation and 1 minute after extubation, with statistical significance (p0 05). Both HR groups showed a significant increase compared to when the medication was discontinued (p0 05). There was no statistically significant difference in the awakening time and postoperative sedation score between the two groups (p0) 05). Conclusion: Intravenous injection of low-dose dexmedetomidine 0.05 mg/kg before the end of surgery can effectively alleviate airway reactions during extubation, without affecting postoperative respiratory movement. It can improve postoperative extubation comfort, reduce stress reactions, and does not affect patient recovery.