目的 探究七氟醚对HT22细胞（小鼠海马神经元细胞系）线粒体Ca2+浓度和线粒体钙单向转运蛋白（MCU）的影响。 方法 使用HT22细胞作为研究对象。HT22细胞采用随机数字表法分为对照组和七氟醚组，对照组细胞暴露于含对照溶剂的培养基，七氟醚组细胞置于含1 mmol/L七氟醚的培养基，两组细胞于37 ℃、5% CO2的培养箱中处理6 h。利用乳酸脱氢酶试剂盒评估细胞损伤情况，细胞增殖检测试剂盒评估细胞活力，利用细胞Ca2+探针Calbryte‑520 AM和Rhod‑2 AM评估细胞质和线粒体Ca2+浓度，利用线粒体探针PK Mito Deep Red进行线粒体定位，应用免疫印迹法检测MCU表达。 结果 与对照组比较，七氟醚组HT22细胞活力降低（P<0.05）、细胞损伤加重（P<0.05）、细胞质和线粒体的Ca2+浓度明显升高（P<0.05），但MCU表达水平差异无统计学意义（P>0.05）。 结论 七氟醚可以引起线粒体钙超载，但不影响MCU表达水平。
Objective To investigate the effect of sevoflurane on mitochondrial Ca2+ concentration and mitochondrial calcium uniporter (MCU) in HT22 cells (mouse hippocampal neuronal cell line). Methods HT22 cells were used for the study. According to the random number table method, HT22 cells were divided into two groups: a control group and a sevoflurane group. Cells in the control group were exposed to the medium containing control solvent, while the sevoflurane group was placed in medium containing 1 mmol/L sevoflurane. All the cells were cultivated an incubator at 37 ℃ and 5% CO2 for 6 h. The cell damage was detected by lactate dehydrogenase kit. The cell viability was assessed by cell proliferation assay kit. The cytoplasmic and mitochondrial Ca2+ concentrations were assessed using the cellular Ca2+ probes Calbryte‑520 AM and Rhod‑2 AM. Mitochondrial localization was performed using the mitochondrial probe PK Mito Deep Red. The expression of MCU was analyzed by Western blot. Results Compared with the control group, the sevoflurane group showed reduced HT22 cell viability (P<0.05), aggravated cell damage (P<0.05), and significantly increased Ca2+ concentrations in the cytoplasm and mitochondria (P<0.05), but no statistical difference was found in MCU expression (P>0.05). Conclusion Sevoflurane can cause mitochondrial calcium overload, but does not affect the MCU expression.
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