Objective To compare the effect of stress response and intraoperative hemodynamic changes by using auditory evoked potential Index (A-line ARX Index, AAI) for judge the depth of anesthesia, versus using the traditional methods such as heart rate and blood pressure. Methods 40 ASA Ⅰ-Ⅱ female patients undergoing laparoscopic surgery were randomly assigned to group A （using AAI method）and group B（control group, using heart rate and blood pressure）by coin flip method. The AAI values of Group A was controlled to maintain the depth of anesthesia in 20±5 AAI. In Group B which using heart rate and blood pressure to adjust the depth of anesthesia and the mean arterial pressure, the heart rate was maintained in the basic value±20%. Five fixed time points were selected to determine the cortisol levels and blood glucose: before induction of anesthesia（T0,baseline）, laryngoscopy and endotracheal intubation（T1）, 5 minutes after CO2 gasless laparoscopic（T2）, 30 minutes after began operating（T3） and extubation （T4）. AAI, hear rate, blood pressure and any clinical signs of inadequate depth of anesthesia such as coughing and lacrimation were recorded at 12 fixed time points during surgery and anesthesia. Results There were no significant differences between the two groups in the general information and general drugs. Open eyes time of the patient in group A（6±5）min were shorter than that in group B（8±6）min (P <0.05). There were two patients in Group B coughing during operation while none in Group A. One patient reported intraoperative awareness in group B while none in Group A. The serum cortisol of two groups had a certain degree of reduction after the start of the anesthesia. Significant difference was found in cortisol level in the patients of group A at time points T3 （146±31）ug/Land T4 （147±33）ug/Lcompared with that at time point T0 （171±31）ug/L( P=0.022，P=0.017). There were also significant differences at time points T3 and T4 between the two groups ( P=0.003，P=0.049). There was downward trend of the blood glucose from induction of anesthesia in group A. There were significant differences in the blood glucose level among the time points T1（4.8±0.7）mmol/L, T2（4.8±0.7）mmol/L and T0（5.5±0.8）mmol/L in group A （P=0.014,P=0.020）, while in group B there were no significant differences. The AAI value of the patients in group A began to decrease significantly after induction, dramatically increased when theCO2 was stopped and seaming the skin. There were significant differences of MAP in the time points P3, P4, P7 between two groups (P <0.05), and there were also significant differences of HR in the time points P2, P3, P4, P10, P11, P12 between two groups (P <0.05). Conclusions Using AAI monitoring in general anesthetic depth control can restrain the stress response caused by CO2 gasless laparoscopic surgery, maintain blood dynamics to stable, and avoid intraoperative awareness.