Objective To summarize the anesthesia experience of video-assisted bilateral pulmonary vein isolation and left atrial appendage exclusion( Wolf Mini－maze procedure) for atrial fibrillation(AF). Methods The clinical files of 14 patients who received Wolf Mini－maze procedure were retrospectively analyzed. All the patients received one lung ventilation (OLV, left and right lung in succession) and intraoperative Eletrophysiologic(EP) Testing. The HR, SBP, CVP, SpO2, Pmax and PETCO2 were monitored and recorded before and at 15 min after OLV, before and during the ablation. Results The average anesthetic time was (4.9±1.5)h, the extubation time in ICU was (9±4)h and the ICU staying time was (72±35)h. After one lung ventilation, the SpO2 decreased while the Pmax increased, especially in the period of left lung ventilation（P<0.05）. The SBP decreased and the SpO2 increased before and during the ablation（P<0.05）. 12 patients were free of AF after the ablation in the operation room, and the other 2 patients retrieve sinus rhythm during the ICU. No patient had serious complications in the postoperative follow-up. Conclusion The variability of respiratory function and the hemodynamics could be found during the Wolf Mini－maze procedure. The anesthesiologist should be familiar with the surgical procedure and the EP testing, maintain the intraoperative hemodynamic stability and treat the hypoxemia during one lung ventilation.